dos.5. California 2+ imaging in the neonatal OHCs
dos.5. California 2+ imaging in the neonatal OHCs The fresh new rat Ocm gene (sequence in the Contour S01) is actually cloned for the pcDNA3.1CFP and you will pEGFP-N1 Flag (Addgene #60360). Then, EGFP-Flag try removed using BamHI (NEB) and you may NotI (NEB) and you can substituted for the brand new mCherry series (gene …
dos.5. California 2+ imaging in the neonatal OHCs Read More »